| Place | Bib Number | Name | Affiliation | Time |
| 1 | 661 | Laura Gresik | Windham High School | 51.08 |
| 2 | 660 | Vanessa Rallis | Windham High School | 51.71 |
| 3 | 494 | Katie Hughes | Edward Little High School | 52.28 |
| 4 | 761 | Jami-Lin Williams | Waterville High School | 53.14 |
| 5 | 41 | Bethany Dumas | Cony High School | 53.19 |
| 6 | 97 | Holly White | Lawrence High School | 53.73 |